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Journal of Clinical Microbiology, January 2003, p. 453-455, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.453-455.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Balamuthia mandrillaris by PCR Assay Using the Mitochondrial 16S rRNA Gene as a Target

Gregory C. Booton,1,2* Jennifer R. Carmichael,1 Govinda S. Visvesvara,3 Thomas J. Byers,1 and Paul A. Fuerst1,2

Department of Molecular Genetics,1 Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, Ohio,2 Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 14 August 2002/ Returned for modification 19 October 2002/ Accepted 27 October 2002

Balamuthia mandrillaris is an opportunistic pathogen that causes granulomatous amebic meningoencephalitis in animals, including humans. Based on sequence analysis of mitochondrial small-subunit-rRNA genes, we developed primers that amplify a Balamuthia-specific PCR product. These primers will be useful for retrospective analyses of fixed tissues and possible identification of Balamuthia in vivo.

* Corresponding author. Mailing address: Department of Molecular Genetics, The Ohio State University, 484 West Twelfth Ave., Columbus, OH 43210-1292. Phone: (614) 292-4570. Fax: (614) 292-4466. E-mail: booton.1{at}osu.edu.

Journal of Clinical Microbiology, January 2003, p. 453-455, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.453-455.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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