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Journal of Clinical Microbiology, January 2003, p. 492-494, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.492-494.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of MicroScan Autoscan for Identification of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients

Lisa Saiman,1* Jane L. Burns,2 Davise Larone,1,{dagger} Yunhua Chen,1 Elizabeth Garber,1 and Susan Whittier3,{ddagger}

Department of Pediatrics, Division of Infectious Diseases, Columbia University,1 Department of Pathology, New York Presbyterian Hospital (Columbia Presbyterian Center), New York, New York,3 Department of Pediatrics, Division of Infectious Diseases, Children's Hospital and Regional Medical Center, University of Washington, Seattle, Washington2

Received 29 March 2002/ Returned for modification 19 June 2002/ Accepted 27 October 2002

Accurate identification of gram-negative bacilli from cystic fibrosis (CF) patients is essential. Only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as Pseudomonas aeruginosa with MicroScan Autoscan. Most common misidentifications were Pseudomonas fluorescens-Pseudomonas putida (i.e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction and yielded the result P. fluorescens/putida) and Alcaligenes spp. Extending the incubation to 48 h improved identification, but 15% of isolates remained misidentified. The MicroScan Autoscan system cannot be recommended for the identification of P. aeruginosa isolates from CF patients.

* Corresponding author. Mailing address: Department of Pediatrics, Division of Infectious Diseases, Columbia University, 630 West 168th St., PH-4-470, New York, NY 10032. Phone: (212) 305-9446. Fax: (212) 305-9491. E-mail: ls5{at}columbia.edu.

{dagger} Present address: New York Presbyterian Hospital (Weill Cornell Center), New York, N.Y.

{ddagger} Present address: Meridian Health System, Neptune, N.J.

Journal of Clinical Microbiology, January 2003, p. 492-494, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.492-494.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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