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Journal of Clinical Microbiology, January 2003, p. 506-508, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.506-508.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism

D. M. Kuhn,1,2 M. Balkis,2 J. Chandra,2 P. K. Mukherjee,2 and M. A. Ghannoum2*

Division of Infectious Diseases, Department of Medicine, Case Western Reserve University,1 Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 441062

Received 6 June 2002/ Returned for modification 29 September 2002/ Accepted 27 October 2002

Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.

* Corresponding author. Mailing address: Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland, LKSD 5028, 11100 Euclid Ave., Cleveland, OH 44106. Phone: (216) 844-8580. Fax: (216) 844-1076. E-mail: mag3{at}po.cwru.edu.

Journal of Clinical Microbiology, January 2003, p. 506-508, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.506-508.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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