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Journal of Clinical Microbiology, October 2003, p. 4512-4520, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4512-4520.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Distribution of Bovine Herpesvirus Type 5 DNA in the Central Nervous Systems of Latently, Experimentally Infected Calves

Fernanda Silveira Flôres Vogel,1 Luizinho Caron,1,{dagger} Eduardo Furtado Flores,1* Rudi Weiblen,1 Evandro Reinoldo Winkelmann,1 Sandra Vanderli Mayer,1 and Reginaldo Gaspar Bastos2

Departamento de Medicina Veterinária Preventiva e Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria,1 Center for Biotechnology, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil2

Received 10 February 2003/ Returned for modification 27 May 2003/ Accepted 27 July 2003

Bovine herpesvirus type 5 (BHV-5) is an alphaherpesvirus associated with meningoencephalitis, a disease highly prevalent in South America. In this study, we investigated the distribution of BHV-5 DNA in the brains of latently, experimentally infected calves by using a PCR for the glycoprotein B gene. Twelve calves inoculated intranasally with a Brazilian BHV-5 isolate were divided into two groups: group A calves (n = 4) were euthanized 55 days postinoculation (p.i.) for tissue collection; group B calves (n = 8) were submitted to dexamethasone administration at day 60 p.i. for reactivation of latent infection and were euthanized 50 days later. Latent infection was reactivated in all group B calves, as demonstrated by virus isolation from nasal secretions and/or seroconversion. Three calves developed neurological disease and died or were euthanized in extremis. For group A calves, viral DNA was consistently detected in the trigeminal ganglia (4/4), midbrain (4/4), thalamus (4/4), and olfactory cortex (4/4) and less frequently in the pons (3/4), cerebellum (3/4), anterior cerebral cortex (2/4), and olfactory bulb (2/4). For calves previously submitted to reactivation (group B), viral DNA was detected with roughly the same frequency in the same areas as for the group A calves. In addition, viral DNA was detected in the posterior (5/5) and dorso-lateral cortex (3/5). All DNA-positive tissues were negative for infectivity and viral antigens. These results demonstrated that latent BHV-5 DNA is present in several areas of the brain during latent infection and that virus reactivation may result in the establishment of latent infection in additional sites of the brain.

* Corresponding author. Mailing address: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil 97105-900. Phone: (011)5555-2208055. Fax: (011)5555-2208034. E-mail: Flores{at}ccr.ufsm.br.

{dagger} Present address: Plum Island Animal Disease Center, Greenport, NY.

Journal of Clinical Microbiology, October 2003, p. 4512-4520, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4512-4520.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Meurens, F., Keil, G. M., Muylkens, B., Gogev, S., Schynts, F., Negro, S., Wiggers, L., Thiry, E. (2004). Interspecific Recombination between Two Ruminant Alphaherpesviruses, Bovine Herpesviruses 1 and 5. J. Virol. 78: 9828-9836 [Abstract] [Full Text]  


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