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Journal of Clinical Microbiology, October 2003, p. 4537-4541, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4537-4541.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Madurella mycetomatis Strains from Mycetoma Lesions in Sudanese Patients Are Clonal

Abdalla Ahmed,1 Wendy van de Sande,2 Henri Verbrugh,2 Ahmed Fahal,1 and Alex van Belkum2*

Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan,1 Erasmus MC, Department of Medical Microbiology and Infectious Diseases, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands2

Received 17 March 2003/ Returned for modification 13 June 2003/ Accepted 17 July 2003

Molecular diversity among clinical isolates of Madurella mycetomatis, the prime fungal agent of human mycetoma in Sudan, could possibly explain the diverse clinical presentations of this severely debilitating infectious disease. In addition, culture-independent DNA-mediated typing tests need to be developed for this organism, since M. mycetomatis DNA, but not the organism itself, can be identified in soil, the material from which infections are thought to originate. A collection of 38 different clinical M. mycetomatis isolates was characterized by large-scale random amplification of polymorphic DNA using 20 different primer species. These analyses, involving at least 2,600 annealing sites, showed a complete lack of DNA fingerprint variation among the various isolates. From the resulting homogeneous DNA fingerprints, seven fragments were cloned and sequenced, and novel, species-specific PCR restriction fragment length polymorphism (RFLP) tests were designed. The seven PCR RFLP tests were successfully performed on the 38 different M. mycetomatis strains. However, again all M. mycetomatis DNA patterns obtained appeared to be identical, whereas patterns produced using DNAs from other fungal species were clearly discriminatory. These results suggest that there is little genetic variation among clinically relevant M. mycetomatis strains from Sudan. The data tentatively imply that different manifestations of mycetoma are due to differences in host susceptibility rather than differential virulence of the causative agent.

* Corresponding author. Mailing address: Erasmus MC, Department of Medical Microbiology and Infectious Diseases, University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 00-31-10-4635813. Fax: 00-31-10-4633875. E-mail: a.vanbelkum{at}erasmusmc.nl.

Journal of Clinical Microbiology, October 2003, p. 4537-4541, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4537-4541.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Desnos-Ollivier, M., Bretagne, S., Dromer, F., Lortholary, O., Dannaoui, E. (2006). Molecular identification of black-grain mycetoma agents.. J. Clin. Microbiol. 44: 3517-3523 [Abstract] [Full Text]  
  • van de Sande, W. W. J., Janse, D.-J., Hira, V., Goedhart, H., van der Zee, R., Ahmed, A. O. A., Ott, A., Verbrugh, H., van Belkum, A. (2006). Translationally Controlled Tumor Protein from Madurella mycetomatis, a Marker for Tumorous Mycetoma Progression. J. Immunol. 177: 1997-2005 [Abstract] [Full Text]  
  • van de Sande, W. W. J., Gorkink, R., Simons, G., Ott, A., Ahmed, A. O. A., Verbrugh, H., van Belkum, A. (2005). Genotyping of Madurella mycetomatis by Selective Amplification of Restriction Fragments (Amplified Fragment Length Polymorphism) and Subtype Correlation with Geographical Origin and Lesion Size. J. Clin. Microbiol. 43: 4349-4356 [Abstract] [Full Text]  


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