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Journal of Clinical Microbiology, October 2003, p. 4578-4582, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4578-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC ß-Lactamase, CMY-2

Johann D. D. Pitout,1* Mark D. Reisbig,2 Mike Mulvey,3 Linda Chui,4 Marie Louie,4 Larry Crowe,5 Deirdre L. Church,1 Sameer Elsayed,1 Dan Gregson,1 Rafiq Ahmed,3 Peter Tilley,6 and Nancy D. Hanson2

Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary,1 Food Borne Illness Investigations, Environmental Health, Calgary Health Region,5 Provincial Laboratory for Public Health, Calgary, Alberta,6 Provincial Laboratory for Public Health (Microbiology) and Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta,4 National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Health Canada, Winnipeg, Manitoba, Canada,3 Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska2

Received 13 February 2003/ Returned for modification 1 May 2003/ Accepted 11 June 2003

Resistance to the extended-spectrum cephalosporins can occur in Salmonella species via the production of extended-spectrum and AmpC ß-lactamases. We describe human infections with Salmonella enterica serotype Newport phage type 14 strains resistant to ceftazidime (CAZ) and cefoxitin (FOX) related to the handling of pet treats containing dried beef. These strains were isolated from five patients in Calgary, Alberta, Canada, during 2002 and were compared to a strain cultured from a commercial pet treat present at the property of one of the patients. The strains were resistant to FOX, CAZ, cefpodoxime, ampicillin, and chloramphenicol; intermediate resistant to ceftriaxone and cefotaxime; and sensitive to the aminoglycosides, ciprofloxacin, cefepime, and imipenem. Isoelectric focusing, multiplex PCR, and sequencing of the amplicons showed that all strains produced the plasmid-encoded AmpC ß-lactamase, CMY-2. Restriction analysis of plasmid DNA following transformation demonstrated that blaCMY-2 was encoded on an approximately 140-kb plasmid. Pulsed-field gel electrophoresis showed the human and pet treat Salmonella strains to be highly related. This study is the first to implicate the transfer of multidrug-resistant Salmonella species through the handling of commercial pet treats containing animal products. In addition to documenting the first cases of human infection caused by CMY-2-producing S. enterica serotype Newport strains in Canada, this study illustrates the necessity of rapid and accurate laboratory-based surveillance in the identification of novel types of antimicrobial resistance.

* Corresponding author. Mailing address: Division of Microbiology, Calgary Laboratory Services, and Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta T3C 0J9, Canada. Phone: (403) 209-5309. Fax: (403) 209-5347. E-mail: johann.pitout{at}cls.ab.ca.

Journal of Clinical Microbiology, October 2003, p. 4578-4582, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4578-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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