This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hurtle, W.
Right arrow Articles by Norwood, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hurtle, W.
Right arrow Articles by Norwood, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2003, p. 4758-4766, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4758-4766.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Denaturing High-Performance Liquid Chromatography To Identify Bacillus anthracis by Analysis of the 16S-23S rRNA Interspacer Region and gyrA Gene

William Hurtle,1 Elizabeth Bode,2 Rebecca Susan Kaplan,2 Jeff Garrison,3 Brian Kearney,2 David Shoemaker,2 Erik Henchal,2 and David Norwood2*

Clinical Research Management, North Royalton, Ohio 44133,1 United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21701-5011,2 Battelle, Columbus, Ohio 432013

Received 5 May 2003/ Returned for modification 16 June 2003/ Accepted 7 July 2003

Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a method for identifying Bacillus anthracis by analyzing two chromosomal targets, the 16S-23S intergenic spacer region (ISR) and the gyrA gene. The 16S-23S ISR was analyzed by this method with 42 strains of B. anthracis, 36 strains of Bacillus cereus, and 12 strains of Bacillus thuringiensis; the gyrA gene was analyzed by this method with 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis. Two blind panels of 45 samples each were analyzed to evaluate the potential diagnostic capability of this method. Our results show that DHPLC is an efficient method for the identification of B. anthracis.

* Corresponding author. Mailing address: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, MD 21702. Phone: (301) 616-4721. Fax: (301) 619-2492. E-mail: David.Norwood{at}det.amed.army.mil.

Journal of Clinical Microbiology, October 2003, p. 4758-4766, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4758-4766.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jury, F., Al-Mahrous, M., Apostolou, M., Sandiford, S., Fox, A., Ollier, W., Upton, M. (2006). Rapid cost-effective subtyping of meticillin-resistant Staphylococcus aureus by denaturing HPLC.. J Med Microbiol 55: 1053-1060 [Abstract] [Full Text]  
  • Posteraro, P., Branca, G., Sanguinetti, M., Ranno, S., Cammarota, G., Rahimi, S., De Carlo, M., Posteraro, B., Fadda, G. (2006). Rapid detection of clarithromycin resistance in Helicobacter pylori using a PCR-based denaturing HPLC assay. J Antimicrob Chemother 57: 71-78 [Abstract] [Full Text]  
  • Xu, L., Ensor, V., Gossain, S., Nye, K., Hawkey, P. (2005). Rapid and simple detection of blaCTX-M genes by multiplex PCR assay. J Med Microbiol 54: 1183-1187 [Abstract] [Full Text]  
  • Randall, L. P., Coldham, N. G., Woodward, M. J. (2005). Detection of mutations in Salmonella enterica gyrA, gyrB, parC and parE genes by denaturing high performance liquid chromatography (DHPLC) using standard HPLC instrumentation. J Antimicrob Chemother 56: 619-623 [Abstract] [Full Text]  
  • McAuliffe, L., Ellis, R. J, Lawes, J. R, Ayling, R. D, Nicholas, R. A. (2005). 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 54: 731-739 [Abstract] [Full Text]  
  • Hurtle, W., Bode, E., Kulesh, D. A., Kaplan, R. S., Garrison, J., Bridge, D., House, M., Frye, M. S., Loveless, B., Norwood, D. (2004). Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe. J. Clin. Microbiol. 42: 179-185 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»