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Journal of Clinical Microbiology, October 2003, p. 4787-4789, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4787-4789.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differentiation of Candida dubliniensis from Candida albicans on Pal's Agar

Asmaa Al Mosaid, Derek J. Sullivan, and David C. Coleman*

Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland

Received 19 May 2003/ Returned for modification 15 July 2003/ Accepted 28 July 2003

Production of a hyphal fringe around colonies grown on Pal's agar (sunflower seed agar) at 30°C for 48 to 72 h provides a simple means of discriminating between isolates of C. dubliniensis and C. albicans with 100% accuracy. Of 128 C. dubliniensis isolates tested on this medium, all produced a hyphal fringe. In contrast, none of the 124 C. albicans isolates tested produced a hyphal fringe. Pal's medium has the added advantage of being prepared from inexpensive, readily available seeds.

* Corresponding author. Mailing address: Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland. Phone: 353 1 6127276. Fax: 353 1 6127295. E-mail: dcoleman{at}dental.tcd.ie.

Journal of Clinical Microbiology, October 2003, p. 4787-4789, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4787-4789.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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