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Journal of Clinical Microbiology, October 2003, p. 4796-4798, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4796-4798.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Optimization of Real-Time PCR Assay for Rapid and Sensitive Detection of Eubacterial 16S Ribosomal DNA in Platelet Concentrates

Tamimount Mohammadi,1,2 Henk W. Reesink,1 Christina M. J. E. Vandenbroucke-Grauls,2 and Paul H. M. Savelkoul2*

Sanquin Blood Bank North West Region,1 Department of Medical Microbiology and Infection Control, VU University Medical Center Amsterdam, Amsterdam, The Netherlands2

Received 1 April 2003/ Returned for modification 19 May 2003/ Accepted 5 July 2003

A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infection Control, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. Phone: 31 20 4440552. Fax: 31 20 4440473. E-mail: P.Savelkoul{at}vumc.nl.

Journal of Clinical Microbiology, October 2003, p. 4796-4798, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4796-4798.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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