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Journal of Clinical Microbiology, October 2003, p. 4805-4807, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4805-4807.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of PCR Assay to Culture for Surveillance Detection of Vancomycin-Resistant Enterococci

Suzanne M. Paule,1* William E. Trick,2 Fred C. Tenover,2 Mary Lankford,1 Susan Cunningham,1 Valentina Stosor,3 Ralph L. Cordell,2 and Lance R. Peterson3

Northwestern Memorial Hospital,1 Feinberg School of Medicine, Northwestern University, Chicago, Illinois,3 Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 13 May 2003/ Returned for modification 1 July 2003/ Accepted 29 July 2003

Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Evanston Hospital, 2650 Ridge Ave., Evanston, IL 60201. Phone: (847) 570-2734. Fax: (847) 733-5314. E-mail: spaule{at}enh.org.

Journal of Clinical Microbiology, October 2003, p. 4805-4807, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4805-4807.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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