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Journal of Clinical Microbiology, October 2003, p. 4812-4814, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4812-4814.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Timothy J. J. Inglis,1* Max Aravena-Roman,1 Simon Ching,2 Kevin Croft,3 V. Wuthiekanun,4 and Brian J. Mee5

Division of Microbiology and Infectious Diseases,1 Division of Clinical Pathology, Western Australian Centre for Pathology and Medical Research,2 Microbiology, School of Biomedical and Chemical Sciences, University of Western Australia, Nedlands,5 Department of Medicine, University of Western Australia, Perth, Western Australia, Australia,3 Wellcome Tropical Diseases Research Unit, Mahidol University, Bangkok, Thailand4

Received 4 June 2003/ Returned for modification 7 July 2003/ Accepted 14 July 2003

Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

* Corresponding author. Mailing address: Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, WA 6909, Australia. Phone: 618 9346 3461. Fax: 618 9381 7139. E-mail: tim.inglis{at}health.wa.gov.au.

Journal of Clinical Microbiology, October 2003, p. 4812-4814, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4812-4814.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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