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Journal of Clinical Microbiology, October 2003, p. 4829-4832, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4829-4832.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved PCR for Detection of the Highly Leukotoxic JP2 Clone of Actinobacillus actinomycetemcomitans in Subgingival Plaque Samples

Knud Poulsen,1* Oum-Keltoum Ennibi,2 and Dorte Haubek3

Department of Medical Microbiology and Immunology,1 Department of Community Oral Health and Pediatric Dentistry, University of Aarhus, DK-8000 Aarhus C, Denmark,3 Dental Faculty, University of Rabat, Rabat, Morocco2

Received 7 April 2003/ Returned for modification 8 June 2003/ Accepted 26 June 2003

The JP2 clone of Actinobacillus actinomycetemcomitans is associated with early-onset periodontitis in certain ethnic populations of African origin. Here, we describe and evaluate a set of primers for PCR to assay for the presence of A. actinomycetemcomitans and to discriminate between JP2-like strains and other genotypes in subgingival plaque samples.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark. Phone: 45 89421736. Fax: 45 86196128. E-mail: kp {at}microbiology.au.dk.

Journal of Clinical Microbiology, October 2003, p. 4829-4832, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4829-4832.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Haubek, D., Ennibi, O.-K., Vaeth, M., Poulsen, S., Poulsen, K. (2009). Stability of the JP2 Clone of Aggregatibacter actinomycetemcomitans. JDR 88: 856-860 [Abstract] [Full Text]  
  • Seki, M., Poulsen, K., Haubek, D., Kilian, M. (2008). Novel Loop-Mediated Isothermal Amplification Method for Detection of the JP2 Clone of Aggregatibacter actinomycetemcomitans in Subgingival Plaque. J. Clin. Microbiol. 46: 1113-1115 [Abstract] [Full Text]  
  • Haubek, D., Poulsen, K., Kilian, M. (2007). Microevolution and Patterns of Dissemination of the JP2 Clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans. Infect. Immun. 75: 3080-3088 [Abstract] [Full Text]  
  • Eriksen, K. T., Haubek, D., Poulsen, K. (2005). Intragenomic recombination in the highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans. Microbiology 151: 3371-3379 [Abstract] [Full Text]  
  • Haubek, D., Ennibi, O.-K., Poulsen, K., Benzarti, N., Baelum, V. (2004). The Highly Leukotoxic JP2 Clone of Actinobacillus actinomycetemcomitans and Progression of Periodontal Attachment Loss. JDR 83: 767-770 [Abstract] [Full Text]  


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