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Journal of Clinical Microbiology, October 2003, p. 4865-4869, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4865-4869.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Photographic and Luminometric Detection of Luciferase Reporter Phages for Drug Susceptibility Testing of Clinical Mycobacterium tuberculosis Isolates

Manzour Hernando Hazbón,1* Nora Guarín,1 Beatriz Eugenia Ferro,1 Ana Lucía Rodríguez,1 Luz Angela Labrada,1 Rafael Tovar,1 Paul F. Riska,2 and William R. Jacobs Jr.3

Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Cali, Colombia,1 Department of Medicine, SUNY Downstate Medical Center, Brooklyn, New York,2 Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York3

Received 19 March 2003/ Returned for modification 11 May 2003/ Accepted 27 July 2003

Luciferase reporter phages (LRPs) have proven to be efficient tools for drug susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higher sensitivity and quantitative results, while a Polaroid film detection method offers a "low-tech" inexpensive alternative that is called the Bronx box. In this work we evaluated, improved, and compared the performance of the luminometer and the Bronx box formats for drug susceptibility testing with LRPs by using 51 clinical isolates of M. tuberculosis, with the agar proportion method (PM) serving as reference. The sensitivity in detecting resistance to isoniazid and rifampin, antibiotics that define multidrug resistance (MDR), was 100% for both methods. The turnaround time for results was reduced from 3 weeks for PM to 54 or 94 h for luminometry or the Bronx box, respectively. These results support the utility of LRPs as a screening test for the surveillance of MDR tuberculosis.

* Corresponding author. Present address: The Center for Emerging Pathogens, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave., Newark, NJ 07103. Phone: (973) 972-2175. Fax: (973) 972-7056. E-mail: hazbonmh{at}umdnj.edu.

Journal of Clinical Microbiology, October 2003, p. 4865-4869, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4865-4869.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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