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Journal of Clinical Microbiology, November 2003, p. 5215-5223, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5215-5223.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

Karen Dohmann,1 Birgit Strommenger,1,{dagger} Karen Stevenson,2 Lucía de Juan,3 Janin Stratmann,1 Vivek Kapur,4 Tim J. Bull,5 and Gerald-Friedrich Gerlach1*

Department of Infectious Diseases, Institute for Microbiology, School of Veterinary Medicine Hannover, D-30173 Hannover, Germany,1 International Research Centre, Pentlands Science Park, Moredun Research Institute, Penicuik EH26 OPZ, Scotland,2 Department of Surgery, St. George's Hospital Medical School, London SW17 ORE, United Kingdom,5 Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain,3 Biomedical Genomics Center and Departments of Microbiology and Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota 551084

Received 27 June 2003/ Returned for modification 13 August 2003/ Accepted 20 August 2003

A combination of representational difference analysis and comparative DNA sequencing revealed that four type I (sheep) isolates of Mycobacterium avium subsp. paratuberculosis were differentiated from nine type II (bovine) isolates by the presence of an 11-bp insertion in a novel M. avium subsp. paratuberculosis-specific region of genomic DNA. Further, our studies show that M. avium subsp. paratuberculosis type I isolates contain three type-specific loci that are missing in M. avium subsp. paratuberculosis type II but are present in M. avium subsp. avium. Taken together, the results are consistent with the hypothesis that M. avium subsp. paratuberculosis type I strains are an evolutionary intermediate between M. avium subsp. avium and M. avium subsp. paratuberculosis type II isolates or share a subset of M. avium subsp. avium type-specific loci through horizontal transfer.

* Corresponding author. Mailing address: Tierärztliche Hochschule Hannover, Zentrum für Infektionsmedizin, Institut für Mikrobiologie. Phone: (49) 511 856-7598. Fax: (49) 511 856-7697. E-mail: gfgerlach{at}gmx.de.

{dagger} Present address: Robert Koch Institut, D-38855 Wernigerode, Germany.

Journal of Clinical Microbiology, November 2003, p. 5215-5223, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5215-5223.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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