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Journal of Clinical Microbiology, November 2003, p. 5224-5226, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5224-5226.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Genotyping Large Numbers of Escherichia coli Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR

Kiersten J. Meacham, Lixin Zhang, Betsy Foxman, Richard J. Bauer, and Carl F. Marrs*

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109

Received 8 April 2003/ Returned for modification 8 July 2003/ Accepted 10 August 2003

We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of bacterial isolates via computer software.

* Corresponding author. Mailing address: Department of Epidemiology, School of Public Health, University of Michigan, 109 S. Observatory, Ann Arbor, MI 48109. Phone: (734) 647-2407. Fax: (734) 764-3192. E-mail: cfmarrs{at}umich.edu.

Journal of Clinical Microbiology, November 2003, p. 5224-5226, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5224-5226.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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