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Journal of Clinical Microbiology, November 2003, p. 5245-5249, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5245-5249.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR

Robert M. Wadowsky,1,2,3* Stella Laus,3 Michael Green,3,4,5 Steven A. Webber,3,4 and David Rowe2

Departments of Pathology,1 Pediatrics,4 Surgery, School of Medicine,5 Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh,2 Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 152133

Received 2 April 2003/ Returned for modification 19 July 2003/ Accepted 5 August 2003

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r2 > 0.900), whereas the plasma and PBL loads correlated poorly (r2 = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

* Corresponding author. Mailing address: Department of Pathology, Children's Hospital of Pittsburgh, 3705 Fifth Ave., Pittsburgh, PA 15213-2583. Phone: (412) 692-5314. Fax: (412) 692-6550. E-mail: Robert.Wadowsky{at}chp.edu.

Journal of Clinical Microbiology, November 2003, p. 5245-5249, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5245-5249.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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