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Journal of Clinical Microbiology, November 2003, p. 5250-5253, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5250-5253.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Restriction Enzyme Analysis of Ribosomal DNA Shows that Candida inconspicua Clinical Isolates Can Be Misidentified as Candida norvegensis with Traditional Diagnostic Procedures

L. Majoros,1* G. Kardos,1 Á. Belák,2 A. Maráz,2 L. Asztalos,3 E. Csánky,4 Z. Barta,5 and B. Szabó1

Department of Medical Microbiology,1 1st Department of Surgery,3 Department of Pulmonology,4 3rd Department of Internal Medicine, Medical and Health Science Center, University of Debrecen, Debrecen,5 Department of Microbiology and Biotechnology, Faculty of Food Science, St. István University, Budapest, Hungary2

Received 20 February 2003/ Returned for modification 24 April 2003/ Accepted 28 July 2003

We identified 29 yeast isolates from 22 patients using the API ID32C panel. Twenty-eight of these isolates were Candida norvegensis and one was C. inconspicua. Although C. norvegensis is considered a pseudohypha-producing species, only one isolate produced pseudohyphae. Restriction enzyme analysis of PCR-amplified ribosomal DNA with four different enzymes proved that all isolates were C. inconspicua.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Debrecen, 4032 Debrecen, Nagyerdei krt. 98, Hungary. Phone: 52-411-717. Fax: 52-414-948. E-mail: major{at}jaguar.dote.hu.

Journal of Clinical Microbiology, November 2003, p. 5250-5253, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5250-5253.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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