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Journal of Clinical Microbiology, November 2003, p. 5258-5261, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5258-5261.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Assessment of Laboratory Performance of Nucleic Acid Amplification Tests for Detection of Mycobacterium tuberculosis

John C. Ridderhof,1 Laurina O. Williams,1* Sue Legois,2 Peter A. Shult,2 Beverly Metchock,1 Louise N. Kubista,2 James H. Handsfield,1 Ronald J. Fehd,1 and Pamela H. Robinson1

Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 Wisconsin State Laboratory of Hygiene, Madison, Wisconsin 537062

Received 12 July 2002/ Returned for modification 6 October 2002/ Accepted 22 August 2003

During implementation of the Centers for Disease Control and Prevention's Mycobacterium tuberculosis nucleic acid amplification (NAA) evaluation program, 27.1% of participants used the same biological safety cabinet for NAA and specimen processing; 28.8% reported not using unidirectional workflow. An association between false positives and adverse responses to quality assurance questions (P = 0.04) illustrated the need for following NCCLS recommendations.

* Corresponding author. Mailing address: Division of Laboratory Systems, Centers for Disease Control and Prevention, Mailstop G25, 4770 Buford Highway, NE, Atlanta, GA 30341. Phone: (770) 488-8130. Fax: (770) 488-8275. E-mail: low1{at}cdc.gov.

Journal of Clinical Microbiology, November 2003, p. 5258-5261, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5258-5261.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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