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Journal of Clinical Microbiology, November 2003, p. 5265-5266, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5265-5266.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Collaborative Consensus for Optimized Multilocus Sequence Typing of Candida albicans

M.-E. Bougnoux,1,2 A. Tavanti,3 C. Bouchier,4 N. A. R. Gow,3 A. Magnier,4 A. D. Davidson,3 M. C. J. Maiden,5 C. d'Enfert,1 and F. C. Odds3*

Unité Biologie et Pathogénicité Fongiques, CNRS URA 2172,1 Génopole, Institut Pasteur, Paris,4 Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Ambroise-Paré, Université Versailles Saint-Quentin en Yvelines, Boulogne-Billancourt, France,2 Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen,3 The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford, United Kingdom5

Received 23 July 2003/ Returned for modification 5 August 2003/ Accepted 19 August 2003

A panel of 86 different Candida albicans isolates was subjected to multilocus sequence typing (MLST) in two laboratories to obtain sequence data for 10 published housekeeping gene fragments. Analysis of data for all possible combinations of five, six, seven, eight, and nine of the fragments showed that a set comprising the fragments AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b was the smallest that yielded 86 unique diploid sequence types for the 86 isolates. This set is recommended for future MLST with C. albicans.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom. Phone: 44 1224555828. Fax: 44 0224555828. E-mail: f.odds{at}abdn.ac.uk.

Journal of Clinical Microbiology, November 2003, p. 5265-5266, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5265-5266.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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