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Journal of Clinical Microbiology, November 2003, p. 5313-5316, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5313-5316.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of PCR-Enzyme-Linked Immunosorbent Assay and Real-Time PCR Assay for Diagnosis of an Unusual Case of Cerebral Toxoplasmosis in a Stem Cell Transplant Recipient

Jean Menotti,1* Gustavo Vilela,2 Stéphane Romand,3 Yves Jean-François Garin,1 Lionel Ades,2 Eliane Gluckman,2 Francis Derouin,1 and Patricia Ribaud2

Laboratoire de Parasitologie-Mycologie,1 Service d'Hématologie-Greffe de Moelle, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris,2 Laboratoire de la Toxoplasmose, Institut de Puériculture, Paris, France3

Received 7 April 2003/ Returned for modification 26 May 2003/ Accepted 4 August 2003

A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis, 1, Avenue Claude Vellefaux, 75475 Paris Cedex 10, France. Phone: 33 1 42 49 95 03. Fax: 33 1 42 49 48 03. E-mail: jean.menotti{at}sls.ap-hop-paris.fr.

Journal of Clinical Microbiology, November 2003, p. 5313-5316, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5313-5316.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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