This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuboki, N.
Right arrow Articles by Igarashi, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuboki, N.
Right arrow Articles by Igarashi, I.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2003, p. 5517-5524, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5517-5524.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes

Noritaka Kuboki,1 Noboru Inoue,1* Tatsuya Sakurai,1 Francescopaolo Di Cello,2 Dennis J. Grab,2 Hiroshi Suzuki,1 Chihiro Sugimoto,1 and Ikuo Igarashi1

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan,1 Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, Maryland 212052

Received 25 March 2003/ Returned for modification 6 May 2003/ Accepted 12 September 2003

While PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions with only simple incubators. An added advantage of LAMP over PCR-based methods is that DNA amplification can be monitored spectrophotometrically and/or with the naked eye without the use of dyes. Here we report our conditions for a highly sensitive, specific, and easy diagnostic assay based on LAMP technology for the detection of parasites in the Trypanosoma brucei group (including T. brucei brucei, T. brucei gambiense, T. brucei rhodesiense, and T. evansi) and T. congolense. We show that the sensitivity of the LAMP-based method for detection of trypanosomes in vitro is up to 100 times higher than that of PCR-based methods. In vivo studies in mice infected with human-infective T. brucei gambiense further highlight the potential clinical importance of LAMP as a diagnostic tool for the identification of African trypanosomiasis.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5647. Fax: 81-155-49-5643. E-mail: ircpmi{at}obihiro.ac.jp.

Journal of Clinical Microbiology, December 2003, p. 5517-5524, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5517-5524.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Takagi, H., Itoh, M., Islam, M. Z., Razzaque, A., Ekram, A. R. M. S., Hashighuchi, Y., Noiri, E., Kimura, E. (2009). Sensitive, Specific, and Rapid Detection of Leishmania donovani DNA by Loop-Mediated Isothermal Amplification. Am J Trop Med Hyg 81: 578-582 [Abstract] [Full Text]  
  • Liang, S.-Y., Chan, Y.-H., Hsia, K.-T., Lee, J.-L., Kuo, M.-C., Hwa, K.-Y., Chan, C.-W., Chiang, T.-Y., Chen, J.-S., Wu, F.-T., Ji, D.-D. (2009). Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica. J. Clin. Microbiol. 47: 1892-1895 [Abstract] [Full Text]  
  • Mugasa, C. M., Laurent, T., Schoone, G. J., Kager, P. A., Lubega, G. W., Schallig, H. D. F. H. (2009). Nucleic Acid Sequence-Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples. J. Clin. Microbiol. 47: 630-635 [Abstract] [Full Text]  
  • Nkouawa, A., Sako, Y., Nakao, M., Nakaya, K., Ito, A. (2009). Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species. J. Clin. Microbiol. 47: 168-174 [Abstract] [Full Text]  
  • Pandey, B. D., Poudel, A., Yoda, T., Tamaru, A., Oda, N., Fukushima, Y., Lekhak, B., Risal, B., Acharya, B., Sapkota, B., Nakajima, C., Taniguchi, T., Phetsuksiri, B., Suzuki, Y. (2008). Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients. J Med Microbiol 57: 439-443 [Abstract] [Full Text]  
  • Inacio, J., Flores, O., Spencer-Martins, I. (2008). Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes. J. Clin. Microbiol. 46: 713-720 [Abstract] [Full Text]  
  • Bonne, N., Clark, P., Shearer, P., Raidal, S. (2008). Elimination of false-positive polymerase chain reaction results resulting from hole punch carryover contamination. jvdi 20: 60-63 [Abstract] [Full Text]  
  • Han, E.-T., Watanabe, R., Sattabongkot, J., Khuntirat, B., Sirichaisinthop, J., Iriko, H., Jin, L., Takeo, S., Tsuboi, T. (2007). Detection of Four Plasmodium Species by Genus- and Species-Specific Loop-Mediated Isothermal Amplification for Clinical Diagnosis. J. Clin. Microbiol. 45: 2521-2528 [Abstract] [Full Text]  
  • Boehme, C. C., Nabeta, P., Henostroza, G., Raqib, R., Rahim, Z., Gerhardt, M., Sanga, E., Hoelscher, M., Notomi, T., Hase, T., Perkins, M. D. (2007). Operational Feasibility of Using Loop-Mediated Isothermal Amplification for Diagnosis of Pulmonary Tuberculosis in Microscopy Centers of Developing Countries. J. Clin. Microbiol. 45: 1936-1940 [Abstract] [Full Text]  
  • Reithinger, R., Dujardin, J.-C. (2007). Molecular Diagnosis of Leishmaniasis: Current Status and Future Applications. J. Clin. Microbiol. 45: 21-25 [Full Text]  
  • Deborggraeve, S., Claes, F., Laurent, T., Mertens, P., Leclipteux, T., Dujardin, J. C., Herdewijn, P., Buscher, P. (2006). Molecular dipstick test for diagnosis of sleeping sickness.. J. Clin. Microbiol. 44: 2884-2889 [Abstract] [Full Text]  
  • Okafuji, T., Yoshida, N., Fujino, M., Motegi, Y., Ihara, T., Ota, Y., Notomi, T., Nakayama, T. (2005). Rapid Diagnostic Method for Detection of Mumps Virus Genome by Loop-Mediated Isothermal Amplification. J. Clin. Microbiol. 43: 1625-1631 [Abstract] [Full Text]  
  • Enomoto, Y., Yoshikawa, T., Ihira, M., Akimoto, S., Miyake, F., Usui, C., Suga, S., Suzuki, K., Kawana, T., Nishiyama, Y., Asano, Y. (2005). Rapid Diagnosis of Herpes Simplex Virus Infection by a Loop-Mediated Isothermal Amplification Method. J. Clin. Microbiol. 43: 951-955 [Abstract] [Full Text]  
  • Chappuis, F., Loutan, L., Simarro, P., Lejon, V., Buscher, P. (2005). Options for Field Diagnosis of Human African Trypanosomiasis. Clin. Microbiol. Rev. 18: 133-146 [Abstract] [Full Text]  
  • Poon, L. L. M., Leung, C. S. W., Chan, K. H., Lee, J. H. C., Yuen, K. Y., Guan, Y., Peiris, J. S. M. (2005). Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification. J. Clin. Microbiol. 43: 427-430 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»