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Journal of Clinical Microbiology, December 2003, p. 5695-5698, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5695-5698.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of S. aureus ID, a New Chromogenic Agar Medium for Detection of Staphylococcus aureus

John D. Perry,* Claire Rennison, Lynne A. Butterworth, Andrew L. J. Hopley, and F. Kate Gould

Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom

Received 26 June 2003/ Returned for modification 1 August 2003/ Accepted 5 September 2003

S. aureus ID (bioMérieux, La Balme Les Grottes, France) is a new chromogenic agar medium designed to enable the isolation of staphylococci and the specific identification of Staphylococcus aureus. S. aureus produces green colonies on this medium due to production of {alpha}-glucosidase. To evaluate this medium, a total of 350 wound swabs were cultured onto S. aureus ID, CHROMagar Staph. aureus, and conventional media routinely used in our laboratory. After 18 to 20 h of incubation, 96.8% of strains formed green colonies on S. aureus ID compared with 91.1% of strains forming mauve colonies on CHROMagar Staph. aureus. A total of 94.3% of strains were recovered within 18 to 20 h with conventional media. The sensitivity was increased after 48 h of incubation to 98.7, 96.2, and 95.6% with S. aureus ID, CHROMagar Staph. aureus, and conventional media, respectively. A total of 97.4% of green colonies on S. aureus ID were confirmed as S. aureus compared with 94.4% of mauve colonies on CHROMagar Staph. aureus. We conclude that S. aureus ID is a highly sensitive and specific medium for the isolation and identification of S. aureus from wound swabs.

* Corresponding author. Mailing address: Department of Microbiology, Freeman Hospital, Tyne and Wear NE7 7DN, Newcastle upon Tyne, United Kingdom. Phone: 44 191 2843111, ext. 26691. Fax: 44 191 2231224. E-mail: john.perry{at}nuth.northy.nhs.uk.

Journal of Clinical Microbiology, December 2003, p. 5695-5698, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5695-5698.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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