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Journal of Clinical Microbiology, December 2003, p. 5755-5759, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5755-5759.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Fine-Structure Molecular Typing of Irish Helicobacter pylori Isolates and Their Genetic Relatedness to Strains from Four Different Continents

Ian M. Carroll,1* Niyaz Ahmed,2 Sarah M. Beesley,1 Aleem A. Khan,3 Sheikh Ghousunnissa,2 Colm A. O Moráin,4 and Cyril J. Smyth1

Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2,1 Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland,4 Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam,2 Deccan College of Medical Sciences and Allied Hospitals, Hyderabad, India3

Received 4 March 2003/ Returned for modification 29 May 2003/ Accepted 1 August 2003

Genotyping of 74 Irish Helicobacter pylori isolates was performed at four different loci (vacA signal sequence and mid-region, insertion-deletion polymorphisms at the 3' end of the cag pathogenicity island, and cagA). The predominant vacA alleles and insertion-deletion motifs suggest an ancestral relationship between Irish isolates and either specific East Asian or Northern European strains. In addition, fluorescent amplified fragment length polymorphism-PCR genotyping and phylogenetic analysis of 32 representative Irish H. pylori isolates and 22 isolates from four different continents demonstrated that the Irish H. pylori isolates examined were weakly clonal and showed some association with both European and Asian isolates. These three genotyping techniques show that Irish H. pylori isolates have distinctive features that may have evolved in this insular European population.

* Corresponding author. Mailing address: Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland. Phone: 353 1 6081728. Fax: 353 1 6799294. E-mail: carrolli{at}tcd.ie.

Journal of Clinical Microbiology, December 2003, p. 5755-5759, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5755-5759.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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