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Journal of Clinical Microbiology, December 2003, p. 5781-5782, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5781-5782.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Diagnosis of Severe Acute Respiratory Syndrome (SARS) by Detection of SARS Coronavirus Nucleocapsid Antibodies in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay

Yuling Shi,1,2 Yanping Yi,1 Ping Li,1 Tieji Kuang,3 Linhai Li,2 Mei Dong,3 Qingjun Ma,1 and Cheng Cao1*

Beijing Institute of Biotechnology, Beijing 100850,1 General Hospital of Guangzhou Command of the People's Liberation Army, Guangzhou 510010,2 309 Army Hospital of the People's Liberation Army, Beijing 100083, People's Republic of China3

Received 23 June 2003/ Returned for modification 25 August 2003/ Accepted 2 September 2003

Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.

* Corresponding author. Mailing address: Beijing Institute of Biotechnology, 27 Taiping Rd., P.O. Box 130(8), Beijing 100850, People's Republic of China. Phone: 86-10 68155151. Fax: 86-10 68155151. E-mail: caoc{at}nic.bmi.ac.cn.

Journal of Clinical Microbiology, December 2003, p. 5781-5782, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5781-5782.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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