This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haroche, J.
Right arrow Articles by El Solh, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haroche, J.
Right arrow Articles by El Solh, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2003, p. 586-591, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.586-591.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Clonal Diversity among Streptogramin A-Resistant Staphylococcus aureus Isolates Collected in French Hospitals

Julien Haroche,1 Anne Morvan,1 Marilyne Davi,1 Jeanine Allignet,1 François Bimet,2 and Névine El Solh1*

Staphylococci Unit,1 Collection of the Pasteur Institute, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 29 August 2002/ Returned for modification 10 October 2002/ Accepted 1 November 2002

We analyzed 62 clinical isolates of streptogramin A-resistant (SGAr) Staphylococcus aureus collected between 1981 and 2001 in 14 hospitals located in seven French cities. These isolates, including five with decreased susceptibility to glycopeptides, were distributed into 45 antibiotypes and 38 SmaI genotypes. Each of these genotypes included between 1 and 11 isolates, the SmaI patterns of which differed by no more than three bands. Although numerous clones were identified, we observed the spread of monoclonal isolates either within the same hospital or within hospitals in distinct cities and at large time intervals. Hybridization with probes directed against 10 SGAr genes (vatA, vatB, vatC, vatD, vatE, vgaA, vgaB, vgaAv, vgbA, and vgbB) revealed six patterns: vgaAv (21 isolates), vatA-vgbA (24 isolates), vgaAv-vatB-vgaB (14 isolates), vgaAv-vatA-vgbA (1 isolate), vgaAv-vatA-vgbA-vatB-vgaB (1 isolate), and vgaA (1 isolate). We detected at least one SGAr determinant in all of the tested isolates. vgaAv, which is part of the recently characterized transposon Tn5406, was found in 59.7% of the tested isolates. Of the 16 streptogramin B-susceptible isolates, 14 carried vgaAv alone and were susceptible to the mixtures of streptogramins, whereas the 2 isolates carrying vgaAv-vatB-vgaB were resistant to these mixtures. vatA-vgbA was found on plasmids of the same apparent size in 26 (42%) of the tested clinical isolates from 18 unrelated SmaI genotypes. The possible dissemination of some of the multiple clones characterized in the present study with an expected increased selective pressure of streptogramins following the recent licensing of Synercid (quinupristin-dalfopristin) must be carefully monitored.

* Corresponding author. Mailing address: Unité des Staphylocoques, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: (33) 01-45-68-83-63. Fax: (33) 01-40-61-31-63. E-mail: nelsolh{at}pasteur.fr.

Journal of Clinical Microbiology, February 2003, p. 586-591, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.586-591.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Novotna, G., Janata, J. (2006). A New Evolutionary Variant of the Streptogramin A Resistance Protein, Vga(A)LC, from Staphylococcus haemolyticus with Shifted Substrate Specificity towards Lincosamides. Antimicrob. Agents Chemother. 50: 4070-4076 [Abstract] [Full Text]  
  • Cassone, M., D'Andrea, M. M., Iannelli, F., Oggioni, M. R., Rossolini, G. M., Pozzi, G. (2006). DNA microarray for detection of macrolide resistance genes.. Antimicrob. Agents Chemother. 50: 2038-2041 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Chesneau, O., Ligeret, H., Hosan-Aghaie, N., Morvan, A., Dassa, E. (2005). Molecular Analysis of Resistance to Streptogramin A Compounds Conferred by the Vga Proteins of Staphylococci. Antimicrob. Agents Chemother. 49: 973-980 [Abstract] [Full Text]  
  • Trad, S., Allignet, J., Frangeul, L., Davi, M., Vergassola, M., Couve, E., Morvan, A., Kechrid, A., Buchrieser, C., Glaser, P., El Solh, N. (2004). DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates. J. Clin. Microbiol. 42: 2054-2064 [Abstract] [Full Text]  
  • Dyke, K. G. H. (2003). Staphylococcus research. Microbiology 149: 2697-2699 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»