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Journal of Clinical Microbiology, February 2003, p. 601-607, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.601-607.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Semiautomated Multiplex PCR Assay for Determination of Streptococcus pneumoniae Serotypes and Serogroups

Elliot R. Lawrence,1,{dagger} David B. Griffiths,1 Siobhán A. Martin,2 Robert C. George,2 and Lucinda M. C. Hall1*

Department of Medical Microbiology, Barts and The London School of Medicine and Dentistry, Queen Mary, University of London, London E1 2AD,1 Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom2

Received 22 April 2002/ Returned for modification 25 September 2002/ Accepted 29 October 2002

A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresis with a 7-min run time, and a serotype or serogroup was assigned on the basis of fragment size. The method was used to test 93 clinical isolates, and all isolates of the serotypes concerned were correctly detected. The strategy would allow the detection of multiple serotypes in a single sample. Detection of additional serotypes could be included as capsule locus sequences become available.

* Corresponding author. Mailing address: Department of Medical Microbiology, Barts and The London School of Medicine and Dentistry, Turner Street, London E1 2AD, United Kingdom. Phone: 44 (0)20 7377 7000, ext. 3228. Fax: 44 (0)20 7375 0518. E-mail: l.m.c.hall{at}qmul.ac.uk.

{dagger} Present address: DNA Research Innovations Ltd., Sittingbourne Research Centre, Kent ME9 8PX, United Kingdom.

Journal of Clinical Microbiology, February 2003, p. 601-607, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.601-607.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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