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Journal of Clinical Microbiology, February 2003, p. 813-815, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.813-815.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Helicobacter pylori in Paraffin-Embedded and in Shock-Frozen Gastric Biopsy Samples by Fluorescent In Situ Hybridization

Holger Rüssmann,1* Anne Feydt-Schmidt,2 Kristin Adler,1 Daniela Aust,3 Almuth Fischer,2 and Sibylle Koletzko2

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie,1 Dr. v. Haunersches Kinderspital,2 Pathologisches Institut, Ludwig Maximilians-Universität München, 80336 Munich, Germany3

Received 25 July 2002/ Returned for modification 3 October 2002/ Accepted 31 October 2002

We report on the successful application of fluorescent in situ hybridization for detection of Helicobacter pylori and determination of its clarithromycin susceptibility in formalin-fixed and paraffin-embedded gastric biopsy specimens that had been prepared for pathological examination. This method is useful when results from conventional culturing with antibiotic susceptibility testing are not available.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut, Ludwig Maximilians-Universität, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 0049-89-51605261. Fax: 0049-89-51605223. E-mail: ruessmann{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, February 2003, p. 813-815, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.813-815.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guimaraes, N., Azevedo, N. F., Figueiredo, C., Keevil, C. W., Vieira, M. J. (2007). Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens. J. Clin. Microbiol. 45: 3089-3094 [Abstract] [Full Text]  
  • Megraud, F (2004). H pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut 53: 1374-1384 [Full Text]  


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