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Journal of Clinical Microbiology, February 2003, p. 826-830, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.826-830.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Dermatophyte Species by 28S Ribosomal DNA Sequencing with a Commercial Kit

Béatrice Ninet,1 Isabelle Jan,1 Olympia Bontems,2 Barbara Léchenne,2 Olivier Jousson,2 Renato Panizzon,2 Daniel Lew,1 and Michel Monod2*

Division des Maladies Infectieuses, Hôpital Cantonal Universitaire, 1211 Geneva,1 Service de Dermatologie (DHURDV), Centre Hospitalier Universitaire Vaudois, 1012 Lausanne, Switzerland2

Received 6 May 2002/ Returned for modification 23 September 2002/ Accepted 26 November 2002

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

* Corresponding author. Mailing address: Service de Dermatologie, Laboratoire de Mycologie, BT422, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland. Phone: 41 21 314 0376. Fax: 41 21 314 0378. E-mail: Michel.Monod{at}chuv.hospvd.ch.

Journal of Clinical Microbiology, February 2003, p. 826-830, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.826-830.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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