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Journal of Clinical Microbiology, February 2003, p. 841-844, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.841-844.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Diagnosis of Enterovirus Infection by Genus-Specific PCR and Enzyme-Linked Immunosorbent Assays

Maria E. Craig,1,2,3* Peter Robertson,4 Neville J. Howard,1 Martin Silink,1 and William D. Rawlinson2,5

The Children's Hospital at Westmead, Sydney,1 Virology Research Laboratory, Virology Division,2 Area Serology Laboratory, Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick,4 School of Women’s and Children’s Health,3 Schools of Medical Sciences, Biochemistry and Biomedical Sciences, University of New South Wales, Kensington, New South Wales, Australia5

Received 26 April 2002/ Returned for modification 1 August 2002/ Accepted 30 October 2002

PCR for the diagnosis of enterovirus infections is resource intensive but is increasingly used due to wide availability. Enzyme-linked immunosorbent assays (ELISAs) that detect heterotypical antibodies against enterovirus immunoglobulin M (IgM), IgA, and IgG were compared with reverse transcription-PCR by using primers specific to the 5' untranslated regions of 60 enterovirus species. The ELISAs were less sensitive than the PCR, and only the ELISA for IgM was highly specific. When retrospective diagnosis is important or when specimens are unsuitable for PCR, the ELISA has a limited role if PCR is not available.

* Corresponding author. Mailing address: Department of Paediatrics, St. George Hospital, Gray St., Kogarah, NSW 2217 Australia. Phone: 61 2 9350 3637. Fax: 61 2 9350 3180. E-mail: m.craig{at}unsw.edu.au.

Journal of Clinical Microbiology, February 2003, p. 841-844, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.841-844.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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