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Journal of Clinical Microbiology, February 2003, p. 863-866, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.863-866.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a 5' Fluorogenic Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis{dagger}

Akihiro Yoshida, Nao Suzuki, Yoshio Nakano,* Takahiko Oho, Miki Kawada, and Toshihiko Koga

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan

Received 22 August 2002/ Returned for modification 10 October 2002/ Accepted 30 October 2002

A 5' nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81 92 642 6423. Fax: 81 92 642 6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.

{dagger} This article is respectfully dedicated to the memory of Toshihiko Koga, who died on 14 October 2001.

Journal of Clinical Microbiology, February 2003, p. 863-866, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.863-866.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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