This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okazaki, N.
Right arrow Articles by Whiley, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okazaki, N.
Right arrow Articles by Whiley, R. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2003, p. 877-879, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.877-879.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Observation of Hot-Cold-Hot Hemolysis Exhibited by Group B Streptococci

Norio Okazaki,1 Ro Osawa,2* Rieko Suzuki,1 Takayasu Nikkawa,1 and Robert A. Whiley3

Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Nakao 1-1-1, Asahi-ku, Yokohama 241-0815,1 Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan,2 Department of Oral Microbiology, St. Bartholomew's and Royal London School of Medicine and Dentistry, London E1 2AD, United Kingdom3

Received 16 September 2002/ Returned for modification 23 October 2002/ Accepted 17 November 2002

Six human isolates of group B streptococci (GBS) were cultured on blood agar anaerobically at 37°C for 18 h and then at 4°C for 6 h and reincubated anaerobically at 37°C for 6 h. Three of the strains showed a marked enlargement of the hemolysis zone compared with that obtained after hot-only (37°C for 18 h) or hot-cold (37°C for 18 h and then 4°C for 6 h) treatment. Subsequent broth culture experiments revealed that enhanced hemolytic activity due to hot-cold-hot treatment was observed in all 6 GBS strains when cultured in the presence of starch.

* Corresponding author. Mailing address: Department of Bioscience Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501. Phone and fax: 81-78-803-5804. E-mail: osawa{at}ans.kobe-u.ac.jp.

Journal of Clinical Microbiology, February 2003, p. 877-879, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.877-879.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»