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Journal of Clinical Microbiology, March 2003, p. 1263-1265, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1263-1265.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Ehrlichia spp. in the Blood of Wild White-Tailed Deer in Missouri by PCR Assay and Serologic Analysis

Max Q. Arens,1* Allison M. Liddell,2 Gerald Buening,3 Monique Gaudreault-Keener,4 John W. Sumner,5 James A. Comer,5 Richard S. Buller,1 and Gregory A. Storch1,2,6

Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital,1 Clinical Virology Laboratory, St. Louis Children's Hospital,4 Departments of Medicine,2 Molecular Microbiology, Washington University School of Medicine, St. Louis,6 Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri,3 Centers for Disease Control and Prevention, Atlanta, Georgia5

Received 17 May 2002/ Returned for modification 2 September 2002/ Accepted 15 December 2002

Blood samples collected from wild deer in Missouri in November of 2000 and 2001 were positive by PCR assays for Ehrlichia chaffeensis (50 of 217; 23%), Ehrlichia ewingii (44 of 217; 20%), and Anaplasma species (214 of 217; 99%). Nucleotide sequences of selected amplicons from the assay for anaplasma matched sequences of the white-tailed deer agent. Serologic analysis of 112 deer sampled in 2000 showed a very high prevalence of antibodies to E. chaffeensis (97 of 112; 87%) and a low prevalence of antibodies reactive with Anaplasma phagocytophila (2 of 112; 2%).

* Corresponding author. Mailing address: Department of Pediatrics, Box 8116, Washington University School of Medicine, One Children's Place, St. Louis, MO 63110. Phone: (314) 454-8035. Fax: (314) 454-8020. E-mail: arens{at}kids.wustl.edu.

Journal of Clinical Microbiology, March 2003, p. 1263-1265, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1263-1265.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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