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Journal of Clinical Microbiology, March 2003, p. 1274-1276, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1274-1276.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Construction and Evaluation of Internal Control DNA for PCR Amplification of Chlamydia trachomatis DNA from Urine Samples

Fotini Betsou,* Katy Beaumont, Jean Marie Sueur, and Jeanne Orfila

Biobanque de Picardie, 80000 Amiens, France

Received 25 July 2002/ Returned for modification 12 October 2002/ Accepted 12 December 2002

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.

* Corresponding author. Mailing address: Biobanque de Picardie, 16 rue Fernel, 80000 Amiens, France. Phone: 33322913967. Fax: 33322916954. E-mail: fay.betsou{at}biobanque-picardie.com.

Journal of Clinical Microbiology, March 2003, p. 1274-1276, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1274-1276.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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