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Journal of Clinical Microbiology, March 2003, p. 1295-1298, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1295-1298.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Histoplasma capsulatum from Culture Extracts by Real-Time PCR

Jose Martagon-Villamil,1 Nabin Shrestha,1,2 Mary Sholtis,2 Carlos M. Isada,1,2 Gerri S. Hall,2 Terry Bryne,3 Barbara A. Lodge,3 L. Barth Reller,3 and Gary W. Procop2*

Division of Infectious Diseases,1 Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio,2 Department of Clinical Microbiology, Duke University Medical Center, Durham, North Carolina3

Received 24 September 2002/ Returned for modification 18 November 2002/ Accepted 1 December 2002

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

* Corresponding author. Mailing address: Clinical Microbiology, Cleveland Clinic Foundation/L40, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, March 2003, p. 1295-1298, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1295-1298.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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