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Journal of Clinical Microbiology, March 2003, p. 1311-1315, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1311-1315.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Mycobacterial Species by PCR Sequencing of Quinolone Resistance-Determining Regions of DNA Gyrase Genes

Jean-Noël Dauendorffer, Isabelle Guillemin, Alexandra Aubry, Chantal Truffot-Pernot, Wladimir Sougakoff, Vincent Jarlier, and Emmanuelle Cambau*

Laboratoire de Bactériologie-Hygiène, Faculté de Médecine Pitié-Salpêtrière, Université Paris VI, and Centre National de Référence pour la Résistance des Mycobactéries aux Antituberculeux, Groupe Hospitalier Pitié-Salpêtrière, Assistance publique-Hôpitaux de Paris, Paris, France

Received 10 September 2002/ Returned for modification 25 October 2002/ Accepted 9 December 2002

The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.

* Corresponding author. Mailing address: Faculté de Médecine Pitié-Salpêtrière, 91, Bd de l'Hôpital, 75634 Paris Cedex 13, France. Phone: 33 (0)1 40 77 97 46. Fax: 33 (0)1 45 82 75 77. E-mail: cambau{at}chups.jussieu.fr.

Journal of Clinical Microbiology, March 2003, p. 1311-1315, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1311-1315.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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