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Journal of Clinical Microbiology, March 2003, p. 998-1003, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.998-1003.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Performance Characteristics of Human Immunodeficiency Virus Type 1 (HIV-1) Genotyping Systems in Sequence-Based Analysis of Subtypes Other than HIV-1 Subtype B

Linda L. Jagodzinski,1* John D. Cooley,1 Mark Weber,1 and Nelson L. Michael2

Henry M. Jackson Foundation for the Advancement of Military Medicine,1 Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, Maryland 208502

Received 14 August 2002/ Returned for modification 4 November 2002/ Accepted 8 December 2002

Given the diversity of human immunodeficiency virus type 1 (HIV-1) subtypes and the emergence of subtypes other than HIV-1 subtype B in the United States, genotypic assays must be capable of delivering sequence data on diverse HIV-1 subtypes. We evaluated the performance of Visible Genetics TRUGENE HIV-1 genotyping kit and Applied Biosystems ViroSeq HIV-1 genotyping system on a panel of 34 well-characterized HIV-1 viral stocks (subtypes A through H). Both assays perform well on diverse HIV-1 subtypes despite being designed for HIV-1 subtype B. The TRUGENE assay produced sequence data for 31 isolates but not for one C and two G isolates. The TRUGENE assay using prototype 1.5 RT-PCR primers and the ViroSeq assay were both successful for all variants tested, although five isolates lacked double-strand sequence coverage in the ViroSeq assay. The availability of standardized HIV-1 genotyping kits that perform reliably with all HIV subtypes will facilitate broad implementation of HIV-1 resistance testing.

* Corresponding author. Mailing address: Henry M. Jackson Foundation, 1600 E. Gude Dr., Rockville, MD 20850. Phone: (301) 251-5042. Fax: (301) 762-7460. E-mail: ljagodzinski{at}hivresearch.org.

Journal of Clinical Microbiology, March 2003, p. 998-1003, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.998-1003.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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