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Journal of Clinical Microbiology, April 2003, p. 1512-1519, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1512-1519.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Ribotyping and Restriction Enzyme Analysis for Inter- and Intraspecies Discrimination of Bordetella avium and Bordetella hinzii

Karen B. Register,^* Randy E. Sacco, and Gwen E. Nordholm

Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010

Received 3 October 2002/ Returned for modification 16 December 2002/ Accepted 23 January 2003

Bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. The inability to distinguish isolates has hampered outbreak investigations and prevents a complete understanding of transmission mechanisms. Isolates of Bordetella hinzii, often referred to as B. avium-like or as Alcaligenes faecalis type II prior to 1995, have also been acquired from the respiratory tracts of diseased poultry but are not believed to be pathogenic for birds. Therefore, differentiating between B. avium and B. hinzii is of importance for veterinary diagnostic laboratories. It was recently reported that both PvuII ribotyping and HinfI/DdeI restriction endonuclease analysis (REA) show promise for distinguishing isolates of B. avium and B. hinzii. Here we compare the ability of these techniques to discern inter- and intraspecies differences. While both approaches distinguished numerous types within a species, only REA was sufficiently discriminatory for routine use as an epidemiologic tool. Both techniques clearly distinguish between B. avium and B. hinzii, although the results of ribotyping are more easily interpreted. Ribotyping and REA identified numerous, previously unrecognized B. hinzii strains from a collection of bordetella isolates, including one acquired from a rabbit. This is the first report of B. hinzii isolation from a nonhuman mammalian species. At least some of the newly recognized B. hinzii isolates have been previously reported to cause disease in poults, suggesting that the pathogenicity of this agent for poultry should be more rigorously examined.

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* Corresponding author. Mailing address: Swine Respiratory Diseases Project, USDA/ARS/National Animal Disease Center, P.O. Box 70, 2300 Dayton Rd., Ames, IA 50010. Phone: (515) 663-7700. Fax: (515) 663-7458. E-mail: kregiste{at}nadc.ars.usda.gov.

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Journal of Clinical Microbiology, April 2003, p. 1512-1519, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1512-1519.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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