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Journal of Clinical Microbiology, April 2003, p. 1543-1547, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1543-1547.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

International Typing Study of Toxin A-Negative, Toxin B-Positive Clostridium difficile Variants

Stuart Johnson,1* Susan P. Sambol,1 Jon S. Brazier,2 Michel Delmée,3 V. Avesani,3 Michelle M. Merrigan,1 and Dale N. Gerding1

Department of Medicine, Veterans Administration Chicago Health Care System, Lakeside Division, and Feinberg School of Medicine, Northwestern University, Chicago, Illinois,1 University Hospital of Wales, Cardiff, Wales,2 Université Catholique de Louvain, Brussels, Belgium3

Received 19 September 2002/ Returned for modification 28 November 2002/ Accepted 11 January 2003

Clinically important strains of Clostridium difficile that do not produce toxin A but produce toxin B and are cytotoxic (A-/B+) have been reported from multiple countries. In order to compare the relatedness of these strains, we typed 23 A-/B+ C. difficile isolates from the United Kingdom (6 isolates), Belgium (11 isolates), and the United States (6 isolates) by three well-described typing methods. Restriction endonuclease analysis (REA), PCR ribotyping, and serogrouping differentiated 11, 4, and 3 different strain types, respectively. Twenty-one of the 23 A-/B+ variants had a 1.8-kb truncation of the toxin A gene characteristic of toxinotype VIII strains; 20 of the 21 toxinotype VIII-like strains were PCR type 17. PCR type 17 isolates could be differentiated into two separate strain groups by serogrouping and by REA. REA further discriminated these isolates into eight subgroups (REA types). PCR type 17-serogroup F-REA group CF isolates were recovered from all three countries, and one specific REA type, CF4, was recovered from patients with C. difficile disease in the United Kingdom and the United States. C. difficile A-/B+ variants of apparent clonal origin are widely distributed in Europe and North America.

* Corresponding author. Present address: Research Service (151), Hines VA Hospital, Hines, IL 60141. Phone: (708) 202-7479. Fax: (708) 216-2269. E-mail: sjohnson{at}lumc.edu.

Journal of Clinical Microbiology, April 2003, p. 1543-1547, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1543-1547.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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