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Journal of Clinical Microbiology, April 2003, p. 1651-1655, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1651-1655.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

New Pathogenicity Marker Found in the Plasticity Region of the Helicobacter pylori Genome

Adriana Santos,1,2,3 Dulciene Maria Magalhães Queiroz,2* Armelle Ménard,1 Armelle Marais,1 Gifone Aguiar Rocha,2 Celso Affonso Oliveira,2 Ana Margarida Miguel Ferreira Nogueira,4 Milton Uzeda,3 and Francis Mégraud1

Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, France,1 Laboratory of Research in Bacteriology,2 Department of Pathology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais,4 Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil3

Received 30 September 2002/ Returned for modification 1 November 2002/ Accepted 13 December 2002

Comparison of gastric carcinoma and gastritis isolates showed the presence of genes, probably carcinoma associated (JHP947 and JHP940), that are situated in a Helicobacter pylori genome region (45 kb in J99 and 68 kb in 26695) called the "plasticity region." This region presents a great variability of DNA sequences. We investigated, by PCR, the presence of the JHP940 and JHP947 genes, as well as the presence of a third gene which seems to be associated with gastritis (HP986), on H. pylori strains isolated from 200 Brazilian patients, 79 of whom had gastric carcinomas and 53 of whom had duodenal ulcers, to confirm this association. Gastritis isolates (n = 68) were included as a control. We also evaluated if these genes were related to the virulence-associated cagA genotype. The present methodology did not permit definitive conclusions to be reached regarding the association between the JHP940 gene and gastric carcinoma or between the HP986 gene and gastritis. However, we showed that the JHP947 gene might be implicated in the development of both duodenal ulcer and gastric carcinoma. The presence of the JHP947 gene was associated with the cagA-positive genotype. The JHP947 gene is a novel virulence marker candidate of H. pylori.

* Corresponding author. Mailing address: Faculdade de Medicina, Av. Alfredo Balena, 190/4026, 30130-100, Belo Horizonte, Minas Gerais, Brazil. Phone and fax: 55 31 32742767. E-mail: dqueiroz{at}medicina.ufmg.br.

Journal of Clinical Microbiology, April 2003, p. 1651-1655, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1651-1655.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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