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Journal of Clinical Microbiology, April 2003, p. 1673-1680, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1673-1680.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Tandem Tetramer-Based Microsatellite Fingerprinting for Typing of Proteus mirabilis Strains

Tomasz Cieslikowski,1* Dobroslawa Gradecka,2 Magdalena Mielczarek,2 and Wieslaw Kaca1,2

Centre of Microbiology and Virology, Polish Academy of Sciences,1 Institute of Microbiology and Immunology, University of Lódz, Lódz, Poland2

Received 25 July 2002/ Returned for modification 15 October 2002/ Accepted 7 January 2003

Two microsatellite tandem repeated tetramers, (GACA)4 and (CAAT)4, were used for Proteus mirabilis strain differentiation. The microsatellite-based PCR tests were applied for the examination of interstrain diversity for 87 P. mirabilis strains. Forty-six of the investigated strains were clinical isolates (5 were hospital isolates and 39 were outpatient clinic isolates); 42 strains were derived from the Kauffmann-Perch collection of laboratory strains. Fingerprinting done with the tetramers had a high discrimination ability [0.992 and 0.940 for (GACA)4 and (CAAT)4, respectively]. The distributions of clinical isolates among well-defined laboratory strains, determined by numerical analysis (unweighted pair-group method with arithmetic averages; Dice similarity coefficient), proved their genetic similarity to reference strains in the Kauffmann-Perch collection. This analysis also indicated that it is possible to estimate some phenotypic properties of P. mirabilis clinical isolates solely on the basis of microsatellite fingerprinting.

* Corresponding author. Mailing address: Centre of Microbiology and Virology, Polish Academy of Sciences, Lodowa 106, 93-232 Lódz, Poland. Phone: 48 (42) 6771-245. Fax: 48 (42) 6771-230. E-mail: tcieslik{at}cmiwpan.lodz.pl.

Journal of Clinical Microbiology, April 2003, p. 1673-1680, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1673-1680.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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