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Journal of Clinical Microbiology, April 2003, p. 1681-1686, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1681-1686.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Clinical Value of Specific Immunoglobulin E Detection by Enzyme-Linked Immunosorbent Assay in Cases of Acquired and Congenital Toxoplasmosis

F. Foudrinier,1* I. Villena,1 R. Jaussaud,2 D. Aubert,1 C. Chemla,1 F. Martinot,1 and J. M. Pinon1

Toxoplasmosis Group, Laboratory of Parasitology-Mycology, IFR53, EA 2070, Hôpital Maison Blanche,1 Infectious Diseases Department, Hôpital Robert Debré, Centre Hospitalier Universitaire, 51092 Reims, France2

Received 12 August 2002/ Returned for modification 25 October 2002/ Accepted 27 December 2002

The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adults; pregnant women with recently acquired infection (acute toxoplasmosis); immunocompetent subjects with recently acquired severe infection (active toxoplasmosis) expressed as fever, adenopathies, splenomegaly, pneumonia, meningitis, or disseminated infection; subjects—some of them immunocompromised—whose previously moderate IgG antibody levels rose, suggesting a reactivation of quiescent toxoplasmosis; and infants born to seroconverted mothers and evaluated for diagnosis of congenital infection and therapeutic management. Specific IgE antibodies were never detected in seronegative subjects. They were present in 85.7% of asymptomatic seroconverters and in 100% of seroconverters with overt toxoplasmosis, following two different kinetics: in the former, the specific IgE titer generally presented a brief peak 2 to 3 months postinfection and then fell rapidly, whereas specific IgE persisted at a very high titer for several months in the latter. IgE emerged concomitantly with the increase in IgG during toxoplasmic reactivation. For neonatal diagnosis of congenital toxoplasmosis, IgE was less informative than IgM and IgA (sensitivities, 59.5, 64.3, and 76.2%, respectively) and had a specificity of 91.9%. Nevertheless, simultaneous measurement of the three isotypes at birth improved the diagnostic yield to 81% relative to the combination of IgA and IgM. Emergence of specific IgE during postnatal treatment for congenital toxoplasmosis is a sign of poor adherence or inadequate dosing.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cedex, France. Phone: (33) 03 26 78 42 20. Fax: (33) 03 26 78 73 28. E-mail: ffoudrinier{at}chu-reims.fr.

Journal of Clinical Microbiology, April 2003, p. 1681-1686, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1681-1686.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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