This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarmati, L.
Right arrow Articles by Andreoni, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarmati, L.
Right arrow Articles by Andreoni, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2003, p. 1760-1762, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1760-1762.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Drug-Associated Resistance Mutations in Plasma and Peripheral Blood Mononuclear Cells of Human Immunodeficiency Virus Type 1-Infected Patients for Whom Highly Active Antiretroviral Therapy Is Failing

Loredana Sarmati,1 Emanuele Nicastri,2 Ilaria Uccella,1 Gabriella d'Ettorre,3 Saverio G. Parisi,4 Lucia Palmisano,5 Clementina Galluzzo,5 Ercole Concia,4 Vincenzo Vullo,3 Stefano Vella,5 and Massimo Andreoni1*

Department of Public Health, University of Rome Tor Vergata,1 National Institute of Infectious Diseases, IRCCS L. Spallanzani,2 Department of Infectious Diseases, University of Rome La Sapienza,3 Laboratory of Virology, Istituto Superiore di Sanità, Rome,5 Institute of Immunology and Infectious Diseases, University of Verona, Verona, Italy4

Received 27 June 2002/ Returned for modification 8 August 2002/ Accepted 23 December 2002

In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reverse transcriptase gene. In eight patients, primary drug resistance mutations were detected only in PBMCs.

* Corresponding author. Mailing address: Department of Public Health and Cellular Biology, University ‘Tor Vergata', Via Montpellier 1, 00133. Rome, Italy. Phone and fax: (39-06) 72596873. E-mail: andreoni{at}uniroma2.it.

Journal of Clinical Microbiology, April 2003, p. 1760-1762, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1760-1762.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steegen, K., Luchters, S., Demecheleer, E., Dauwe, K., Mandaliya, K., Jaoko, W., Plum, J., Temmerman, M., Verhofstede, C. (2007). Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots. J. Clin. Microbiol. 45: 3342-3351 [Abstract] [Full Text]  
  • Parisi, S. G., Boldrin, C., Cruciani, M., Nicolini, G., Cerbaro, I., Manfrin, V., Dal Bello, F., Franchin, E., Franzetti, M., Rossi, M. C., Cattelan, A. M., Romano, L., Zazzi, M., Andreoni, M., Palu, G. (2007). Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral-Drug-Naive Patients. J. Clin. Microbiol. 45: 1783-1788 [Abstract] [Full Text]  
  • Monie, D., Simmons, R. P., Nettles, R. E., Kieffer, T. L., Zhou, Y., Zhang, H., Karmon, S., Ingersoll, R., Chadwick, K., Zhang, H., Margolick, J. B., Quinn, T. C., Ray, S. C., Wind-Rotolo, M., Miller, M., Persaud, D., Siliciano, R. F. (2005). A Novel Assay Allows Genotyping of the Latent Reservoir for Human Immunodeficiency Virus Type 1 in the Resting CD4+ T Cells of Viremic Patients. J. Virol. 79: 5185-5202 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»