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Journal of Clinical Microbiology, April 2003, p. 1763-1765, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1763-1765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

DNase Pretreatment of Master Mix Reagents Improves the Validity of Universal 16S rRNA Gene PCR Results

Alexandra Heininger,1 Marlies Binder,1 Andreas Ellinger,2 Konrad Botzenhart,2 Klaus Unertl,1 and Gerd Döring2*

Klinik für Anästhesiologie und Transfusionsmedizin, Abteilung für Anästhesiologie,1 Institut für Allgemeine Hygiene und Umwelthygiene, Eberhard-Karls-Universität Tübingen, D-72074 Tübingen, Germany2

Received 5 July 2002/ Returned for modification 29 September 2002/ Accepted 29 December 2002

DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.

* Corresponding author. Mailing address: Institut für Allgemeine Hygiene und Umwelthygiene, Wilhelmstraße 31, D-72074 Tübingen, Germany. Phone: 0049-7071-2982069. Fax: 0049-7071-293011. E-mail: Gerd.Doering{at}uni-tuebingen.de.

Journal of Clinical Microbiology, April 2003, p. 1763-1765, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1763-1765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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