This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diggle, M. A.
Right arrow Articles by Clarke, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Diggle, M. A.
Right arrow Articles by Clarke, S. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2003, p. 1766-1768, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1766-1768.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of a Fluorescence-Based PCR Method for Identification of Serogroup A Meningococci

M. A. Diggle,1 K. Smith,1 E. K. Girvan,2 and S. C. Clarke1,3*

Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital,1 Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow,3 Department of Microbiology, Wishaw General Hospital, Wishaw, United Kingdom2

Received 21 August 2002/ Returned for modification 2 October 2002/ Accepted 29 December 2002

Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.

* Corresponding author. Mailing address: Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow G21 3UW, United Kingdom. Phone: 44 141 201 3836. Fax: 44 141 201 3663. E-mail: stuart.clarke{at}northglasgow.scot.nhs.uk.

Journal of Clinical Microbiology, April 2003, p. 1766-1768, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1766-1768.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gray, S. J., Trotter, C. L., Ramsay, M. E., Guiver, M., Fox, A. J., Borrow, R., Mallard, R. H., Kaczmarski, E. B. (2006). Epidemiology of meningococcal disease in England and Wales 1993/94 to 2003/04: contribution and experiences of the Meningococcal Reference Unit.. J Med Microbiol 55: 887-896 [Abstract] [Full Text]  
  • Bennett, D. E., Cafferkey, M. T. (2006). Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease.. J. Clin. Microbiol. 44: 1127-1131 [Abstract] [Full Text]  
  • Taha, M.-K., Alonso, J.-M., Cafferkey, M., Caugant, D. A., Clarke, S. C., Diggle, M. A., Fox, A., Frosch, M., Gray, S. J., Guiver, M., Heuberger, S., Kalmusova, J., Kesanopoulos, K., Klem, A.-M., Kriz, P., Marsh, J., Molling, P., Murphy, K., Olcen, P., Sanou, O., Tzanakaki, G., Vogel, U. (2005). Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis. J. Clin. Microbiol. 43: 144-149 [Abstract] [Full Text]  
  • Tsang, R. S. W., Zollinger, W. D. (2005). Serological Specificities of Murine Hybridoma Monoclonal Antibodies against Neisseria meningitidis Serogroups B, C, Y, and W135 and Evaluation of Their Usefulness as Serogrouping Reagents by Indirect Whole-Cell Enzyme-Linked Immunosorbent Assay. CVI 12: 152-156 [Abstract] [Full Text]  
  • Bennett, D. E., Mulhall, R. M., Cafferkey, M. T. (2004). PCR-Based Assay for Detection of Neisseria meningitidis Capsular Serogroups 29E, X, and Z. J. Clin. Microbiol. 42: 1764-1765 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»