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Journal of Clinical Microbiology, April 2003, p. 1779-1782, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1779-1782.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of the BDProbeTec ET System for Direct Detection of Mycobacterium tuberculosis in Pulmonary and Extrapulmonary Samples: a Multicenter Study

Gianna Mazzarelli,1,2 Laura Rindi,3 Paola Piccoli,4 Claudio Scarparo,4 Carlo Garzelli,3 and Enrico Tortoli1*

Microbiology and Virology Laboratory, Regional Reference Center for Mycobacteria,1 Microbiological and Virological Serum-immunology Laboratory, Careggi Hospital, Florence,2 Department of Experimental Pathology, Medical Biotechnologies, Infectivology and Epidemiology, University of Pisa, Pisa,3 Regional Reference Center for Mycobacteria, S. Bortolo Hospital, Vicenza, Italy4

Received 14 October 2002/ Returned for modification 27 November 2002/ Accepted 2 January 2003

We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.

* Corresponding author. Mailing address: Regional Reference Center for Mycobacteria, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Florence, Italy. Phone: 39-055-4279199. Fax: 39-055-4279010. E-mail: e.tortoli{at}libero.it.

Journal of Clinical Microbiology, April 2003, p. 1779-1782, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1779-1782.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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