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Journal of Clinical Microbiology, May 2003, p. 1850-1855, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.1850-1855.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum Organisms in Genitourinary Samples by PCR-Microtiter Plate Hybridization Assay

Takashi Yoshida,1 Shin-Ichi Maeda,2 Takashi Deguchi,3 Takamaro Miyazawa,1 and Hiroaki Ishiko1*

Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Itabashi, Tokyo 174-8555,1 Department of Urology, Toyota Memorial Hospital, Toyota City, Aichi 471-0821,2 Department of Urology, Gifu University School of Medicine, Gifu City, Gifu 500-8705, Japan3

Received 3 July 2002/ Returned for modification 13 August 2002/ Accepted 23 February 2003

We present a method for detecting the presence of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms, which are thought to be associated with nongonococcal urethritis (NGU) and other genitourinary infections, in clinical samples. This method consists of PCR amplification of a part of the 16S rRNA gene followed by 96-well microtiter plate hybridization assay using four species-specific capture probes to detect the targets. To test the efficacy of this method, we applied it to the detection of the four species in the urine of patients with NGU. There were no cross-reactions with other human mycoplasmas or ureaplasmas, and the PCR-microtiter plate hybridization assay detected as few as 10 copies of the 16S rRNA gene of each of the four species. Based on these results, this PCR-microtiter plate hybridization assay can be considered an effective tool for the diagnosis of genitourinary infections with mycoplasmas or ureaplasmas.

* Corresponding author. Mailing address: Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., 3-30-1 Shimura, Itabashi, Tokyo 174-8555, Japan. Phone: 81-3-5994-2431. Fax: 81-3-5994-2972. E-mail: ishiko{at}m1.mbcl.co.jp.

Journal of Clinical Microbiology, May 2003, p. 1850-1855, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.1850-1855.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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