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Journal of Clinical Microbiology, May 2003, p. 2106-2112, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2106-2112.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of a New Variant of Shiga Toxin 1 in Escherichia coli ONT:H19 of Bovine Origin

Christine Bürk,1* Richard Dietrich,1 Gabriele Açar,1 Maximilian Moravek,1 Michael Bülte,2 and Erwin Märtlbauer1

Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, Ludwig-Maximilians-Universität, 80539 Munich,1 Institute for Veterinary Food Science, Faculty of Veterinary Medicine, Justus-Liebig-Universität, 35392 Gießen, Germany2

Received 23 October 2002/ Returned for modification 27 December 2002/ Accepted 25 January 2003

A new variant of Shiga toxin 1 (Stx1), designated Stx1d, which deviates considerably more than any other known variant from Stx1 encoded by phage 933J, was identified in an Escherichia coli strain, ONT:H19, isolated from bovine feces. The complete stx1 gene of this strain was amplified and sequenced. Nucleotide sequence homology with stx1 from phage 933J was only 91%, resulting in the substitution of 20 amino acids in the A subunit and 7 amino acids in the B subunit of the protein. Cell culture supernatant of this strain, which was negative for stx2 by PCR testing, was cytotoxic to Vero cells and gave positive results in two commercial enzyme-linked immunosorbent assays for Stx. PCR primers were constructed for the specific detection of the new variant. The findings of this study suggest that Stx1 is not as conserved as thought before and that there might be more variants which cannot be detected by commonly used PCR methods.

* Corresponding author. Mailing address: Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, Ludwig-Maximilians-Universität, Veterinärstr. 13, 80539 Munich, Germany. Phone: 49 89 2180 2256. Fax: 49 89 2180 3793. E-mail: c.buerk{at}mh.vetmed.uni-muenchen.de.

Journal of Clinical Microbiology, May 2003, p. 2106-2112, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2106-2112.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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