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Journal of Clinical Microbiology, May 2003, p. 2141-2143, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2141-2143.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Fluconazole-Resistant Isolates of Candida glabrata by Using an Agar Screen Assay

Susan M. Nelson1 and Charles P. Cartwright1,2*

Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, Minneapolis, Minnesota 55415,1 Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 554552

Received 10 October 2002/ Returned for modification 14 January 2003/ Accepted 25 January 2003

The ability of a fluconazole-containing agar screen assay to accurately detect isolates of Candida glabrata resistant to the azole antifungal agent fluconazole was evaluated on a collection of 100 clinical isolates of this organism. Results were correlated with the MIC of fluconazole for these isolates and compared with the results of a previously published disk diffusion-based fluconazole resistance screening test. Agar screen assay results were in categorical agreement with MIC-based determinations for 97% (97/100) of the isolates tested. This correlation was higher than that obtained with the disk diffusion technique, which categorized only 87% (87/100) of isolates correctly, and suggests that the agar screening approach can effectively expedite fluconazole susceptibility testing of C. glabrata isolates.

* Corresponding author. Mailing address: Clinical Laboratories, MC #812, Hennepin County Medical Center, 701 Park Ave., Minneapolis, MN 55415. Phone: (612) 347-3026. Fax: (612) 904-4229. E-mail: charles.cartwright{at}co.hennepin.mn.us.

Journal of Clinical Microbiology, May 2003, p. 2141-2143, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2141-2143.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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