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Journal of Clinical Microbiology, May 2003, p. 2170-2173, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2170-2173.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Screening for Streptococcus agalactiae in Vaginal Specimens of Pregnant Women by Fluorescent In Situ Hybridization

Laura A. Artz,* Volkhard A. J. Kempf, and Ingo B. Autenrieth

Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Eberhard-Karls-Universität Tübingen, D-72076 Tübingen, Germany

Received 1 October 2002/ Returned for modification 24 November 2002/ Accepted 27 January 2003

Group B streptococci (GBS) are the most frequent pathogens in neonates with sepsis. A rapid screening method is required to identify carriage of GBS in pregnant women at the time of delivery. In order to detect GBS in vaginal specimens, the efficiency of the standard culture versus fluorescent in situ hybridization (FISH) was investigated. In 258 examined vaginal specimens, FISH identified 58 of the 59 GBS-positive samples (98.3%), whereas by means of standard culture only 38 specimens were positive (64.4%). We recommend FISH as a rapid, specific, highly sensitive screening technique for the detection of GBS in pregnant women at delivery.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Elfriede-Aulhorn-Str. 6, D-72076 Tübingen, Germany. Phone: 0049-7071-2982072. Fax: 0049-7071-293435. E-mail: amelia-laura.artz{at}med.uni-tuebingen.de.

Journal of Clinical Microbiology, May 2003, p. 2170-2173, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2170-2173.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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