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Journal of Clinical Microbiology, May 2003, p. 2180-2183, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2180-2183.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses

James J. Dunn,1,2* Chris Gordon,1 Christy Kelley,1 and Karen C. Carroll1,2,{dagger}

ARUP Laboratories, Inc., Salt Lake City, Utah 84108,1 Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 841322

Received 11 October 2002/ Returned for modification 29 December 2002/ Accepted 25 January 2003

The INFLU A·B-Quick and Directigen Flu A+B enzyme immunoassays were compared with direct immunofluorescence and cell culture for detection of influenza A and B viruses in a total of 255 patient specimens. Both assays identified 23 of 42 influenza A viruses (sensitivity, 54.8%; specificity, 100%; positive predictive value [PPV], 100%; negative predictive value [NPV], 91.8%). The INFLU A·B-Quick assay identified 10 of 16 influenza B viruses (sensitivity, 62.5%; specificity, 99.6%; PPV, 90.9%; NPV, 97.5%), and the Directigen Flu A+B assay detected 9 of 16 influenza B viruses (sensitivity, 56.3%; specificity, 99.6%; PPV, 90%; NPV, 97.1%).

* Corresponding author. Present address: Cook Children's Medical Center, 801 Seventh Ave., Fort Worth, TX 76104. Phone: (817) 870-6475. Fax: (817) 885-4316. E-mail: jdunn{at}cookchildrens.org.

{dagger} Present address: Microbiology Division, Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21087-7093.

Journal of Clinical Microbiology, May 2003, p. 2180-2183, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2180-2183.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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